Next, we generated a ternary input circuit, which responded to light, doxycycline and cumate. We first constructed a dual input circuit converting different light intensities into varying of the sensitivity of the promoter to a chemical inducer (doxycycline). Here, in HEK 293 cells, we present combinatory genetic circuits responding to light and chemical signals, simultaneously.
Moreover, light-inducible systems provide fast and reversible means for spatiotemporal control of gene expression. This is an unusual case of observation of white light emission from a single molecule Sm( III) complex.Multi-signal processing circuits are essential for rational design of sophisticated synthetic systems with good controllability and modularity, therefore, enable construction of high-level networks.
With the variation of the excited wavelength and concentration of the solution, complex 1 shows a tunable white light emission with the balance of three primary colors. In complex 1, partial energy transfer from BTPB results in Sm( III)-based red light emission in addition to the BTPB-based blue/green emission. In some cases aggregation of the ligand results in the appearance of a new luminescence band at about 510 nm in addition to the monomer fluorescence. UV-Vis absorption and emission spectroscopic techniques are used to investigate photophysical properties of the ligand and its complexes in THF and CHCl 3. Reaction of the doubly negatively charged bis-bidentate ligand with lanthanide ions forms triple-stranded dinuclear complexes Sm 2(BTPB) 3(H 2O) 4 ( 1) and Gd 2(BTPB) 3(H 2O) 4 ( 2), which have been fully characterized by various spectroscopic techniques. The ligand bears two benzoyl β-diketonate sites linked by a 1,1′-binaphthoxy spacer. A novel bis-β-diketone ligand, 4,4′-bis(4,4,4-trifluoro-1,3-dioxobutyl)(phenoxy)-1,1′-binaphthalene (BTPB), is designed for synthesis of a white light emissive lanthanide complex.
0 kommentar(er)
